Description
The successful candidate will be working in an interdisciplinary and international team and focus on one of the aspects of ion transport investigated in our group. Our laboratory works on many aspects of ion transport processes, ranging from structure/function analysis and biophysics to cell biology, physiology, and role in human disease. Accordingly we use a broad array of techniques, including molecular biology, electrophysiology, ion imaging, morphology, biochemistry, and generation and analysis of genetically modified mice. We are focusing on CLC chloride channels and transporters, KCNQ potassium channels, and Ca-activated Cl channels. These can be found at the plasma membrane and on intracellular organelles like endosomes and lysosomes. Phenotypes caused by gene disruption in mice or genetic disease in humans include e.g. impaired endocytosis and lysosomal storage, neurodegeneration, epilepsy, and osteopetrosis. Our knock-out mouse models lead to many fascinating problems that can be tackled by electrophysiological analysis of slice preparations or of cultured cells derived from these mice, often in combination with optical methods. In the Ion Imaging field, we would in particular like to study the luminal pH and chloride of vesicles, but also use e.g. Ca-imaging as an indicator of neuronal activity. Our lab is well-equipped in this area with five patch-clamp set-ups, two 2-electrode voltage-clamp set-ups for oocytes, two ion imaging set-ups, TIRF and confocal microscopes.中国-博士人才网发布
