Job Description
Apply in vitro evolution to generate large-scale molecular diversity using the bacterial periplasmic binding protein (PBP) structure as the fundamental science level
Understand the binding mechanisms of the new PBPs and pave the way for the design of biosensors for a much broader range of analytes in future projects
Join CSIRO, Australia’s leading scientific research organisation
It is widely accepted that increased adoption of sensing technologies will transform many industries over the next 50 years. Biosensors will play an important part in this transformation. The Fellow will apply in vitro evolution to generate large-scale molecular diversity using the bacterial PBP structure as the transduction scaffold which is fully compatible with CSIRO’s proprietary Cybertongue device. The Fellow will have access to CSIRO’s supercomputer clusters, state-of-the-art molecular modelling software packages and world class C3 protein crystallography facilities.
Specifically you will:
Develop PBPs with specificities to a number of structurally related nitrogen-based heterocyclic compounds using in vitro evolution
Develop and screen a diverse ‘structure-guided’ library of synthetic PBP sequences
Characterise the binding specificities and signalling characteristics of selected PBPs, define non-selected effects of the introduced amino acid substitutions
Participate in the identification of further opportunities arising from research and initiate new lines of research
Assist in the planning and preparation of research proposals, carry out investigations requiring originality, creativity and innovation
Present results at international conferences, publish in scientific journals for the highest possible impact
Location: Canberra, Australia
Salary: $81k – $88k plus up to 15,4% super
Term: 3 years
Reference Number: ACT13/03727
To be successful, you will have:
A PhD, or will shortly satisfy the requirements for a PhD in a relevant scientific discipline, and no more than 3 years relevant post doctoral experience
Ability to conduct innovative research in protein design, expression and biochemical characterisation
Strong oral and written communication skills including the ability to publish results in scientific journals
Ability to develop experimental plans and pursue novel research approaches
Proven originality, creativity and innovation in problem solving and introducing new directions or approaches
Ability to work with a broad range of people and independently, to meet performance deadlines
Experience with biophysical analysis of proteins, in silico protein modelling or experience with biosensing and biosensors is desirable
About the research group: This project is a collaboration between CSIRO’s Chemosensing and Enzymology groups. The principal supervisor, Dr Stephen Trowell leads CSIRO’s Quality Biosensor research team responsible for developing CYBERNOSE technology. The team comprises of experts in enzyme evolution and engineering: Dr. Colin Scott, biosensor research: Dr. Helen Dacres, protein modelling and in silico design: Dr. Andrew Warden. Dr. Alisha Anderson, who has expersite in biological chemosensing and genetics, will mentor the Fellow. Their expertise offers the Fellow unparalleled opportunity to make ground-breaking discoveries in a rapidly evolving field.
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